Image cytometry as an alternative to flow cytometry for the transplant histocompatibility crossmatch assay
نویسندگان
چکیده
منابع مشابه
Living-donor kidney transplant in T-cell and B-cell flow cytometry crossmatch-positive patients.
OBJECTIVES Complement-dependent cytotoxic crossmatch is an important indicator for kidney transplant. However, there is controversy about treatment for flow cytometry crossmatch-positive cases. MATERIALS AND METHODS This was a retrospective study of 127 living-donor kidney transplant recipients from May 2007 to July 2011. We divided patients into 115 flow cytometry crossmatch T-cell and B-cel...
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Notch signaling is an evolutionary conserved signaling pathway that plays an indispensable role during development, and in the maintenance of homeostatic processes, in a wide variety of tissues (Kopan, 2012; Hori et al., 2013). The multifaceted roles of Notch signaling are stringently regulated at different levels. One of the most important aspects of regulation is the binding of different Notc...
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BACKGROUND The lymphocytes separated from whole blood are used in HLA flow cytometry crossmatch (FCXM) for renal transplantation. In this study, the methodology of whole blood flow cytometry was applied to FCXM, omitting lymphocyte separation step. METHODS In the 20 cases (including positive 5 cases) of T cell FCXM for renal transplantation, the standard assay using the separated mononuclear ...
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Dye exclusion tests are used to determine the number of live and dead cells. These assays are based on the principle that intact plasma membranes in live cells exclude specific dyes, whereas dead cells do not. Although widely used, the trypan blue (TB) exclusion assay has limitations. The dye can be incorporated by live cells after a short exposure time, and personal reliability, related to the...
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Lasers are the principle light sources of flow cytometers. Most modern benchtop instruments feature air-cooled argon-ion laser emitting 10-25 mW of light at 488 nm. This unit covers the various types of lasers available and the qualities that make them suitable or unsuitable for use in flow cytometers. Also included is a discussion of future directions, particularly in the development of solid-...
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ژورنال
عنوان ژورنال: HLA
سال: 2019
ISSN: 2059-2302,2059-2310
DOI: 10.1111/tan.13515